As a new addition to our research on metabolic pathway engineering, we have recently published the results of our research and collaborations in the framework of the ERA-IB TTRaFFIC project on Metabolic engineering and fermentation optimization improve itaconic acid production in Aspergillus niger.
In this paper we show how we have successfully improved our patented IA biosynthesis pathway by metabolic engineering of ATP-citrate lyase. Furthermore, we have improved the fermentation protocol, and combined with the improved pathway this has resulted in titers close to those required for commercial application of this technology. In addition to these results we have been able to show that the strains developed sofar are also able to produce high levels of itaconic acid on agricultural residues based feedstocks (research performed in the framework of the TKI-BBE project Veni Vidi Viride). Parties interested in this technology and the associated IP portfolio are invited to contact us (Cornelis.Mijnders@ddna-biotech.com).