In a recently concluded collaboration between Leiden University and Dutch DNA Biotech, novel biological approaches using fungi were explored to recycle synthetic polymers, in particular from matrasses. This research project provided a perfect industrial fit to the news that The Netherlands is a front-runner in matrass recycling. Students from various Dutch universities contributed both in the lab and in industrial case studies to the project, which was carried out with a Top Sector Energy Subsidy from the Ministry of Economic Affairs.
Our industrious A. niger has joined the centennial club by being capable of producing more than 100 g/L protein titers. Possibly even more exciting than the protein titers is the remarkably low viscosity of our strain; a yeast-like fermentation produces filamentous fungus-like protein titers. This breakthrough in our platform strain will enable Dutch DNA Biotech to facilitate the transition to a bio-based economy in cooperation with our partners.
During the upcoming industrial fermentation conference ‘Recent Advances in Fermentation Technology’ (RAFT), held in Fort Myers, Florida, USA, at the end of October, our fermentation scientist Pascal van Alphen will share more information on these exciting fermentation results of our Aspergillus niger production host.
As a new addition to our research on metabolic pathway engineering, we have recently published the results of our research and collaborations in the framework of the ERA-IB TTRaFFIC project on Metabolic engineering and fermentation optimization improve itaconic acid production in Aspergillus niger.
In this paper we show how we have successfully improved our patented IA biosynthesis pathway by metabolic engineering of ATP-citrate lyase. Furthermore, we have improved the fermentation protocol, and combined with the improved pathway this has resulted in titers close to those required for commercial application of this technology. In addition to these results we have been able to show that the strains developed sofar are also able to produce high levels of itaconic acid on agricultural residues based feedstocks (research performed in the framework of the TKI-BBE project Veni Vidi Viride). Parties interested in this technology and the associated IP portfolio are invited to contact us (Cornelis.Mijnders@ddna-biotech.com).
Peter Punt will be present at the Fungal Genetics Conference 12-17 March, in Pacific Grove, CA/USA. The conference brings together researchers working on all aspects of fungal genetics and encourages communication and collaboration between those interested in genomics, gene regulation, cell biology and development, evolutionary biology, fungal-host interactions, and biotechnology. For more information and to meet Peter: email@example.com.
Although plastics have given a lot to mankind, the world is struggling with the waste.
In the first quarter of next year, Dutch DNA and Leiden University will start a project on the enzymatic recycling of plastic. The project, BACK TO MONO (or in short B2M), aims to deliver new enzymes that can degrade plastics to their original chemical building blocks. The ultimate objective of the project is an industrial process in which those building blocks will be re-used for new products. The project is co-funded by the Dutch Government – RVO approved it this week via its subsidy instrument TKI – and wants to deliver a proof of principle. More concrete, the project aims to deliver a set of enzymes to do this, as well as a production organism to produce these enzymes. With the results of the project Dutch DNA will take steps towards industrial application: Industry using enzymatic degradation of collected plastic waste to feed in current existing value chain with recycled monomers: back to mono and then back to plastics again!
Dutch DNA Biotech delegation – Dr Ebru Alazi, Dr Sylvia Segers, Abeer Hossain and Prof Peter Punt – will join the Mycologische dag of the KNMV at Danone venue on Utrecht Science Park, November 30th.
Don’t miss the presentation of Abeer Hossain starting at 14:00 hours. Abeer is our lead scientist organic acids. He presents the results of his research on Itaconic Acid degradation in Aspergillus Niger.
Dutch DNA Biotech acquired new Eppendorf BioFlo 320 fermentors with double 5 L vessels. By that we expand our fermentation capacity again with state-of-the-art equipment. As Dutch DNA Biotech plans to scale up its fermentation activities further (routinely performing a minimum of 8 fermentations each week / year round) these assets are more than welcome. With currently 20 5 L fermentors DDNA will reach more milestones in both protein as well as biochemical production technology developments
Our CEO, Art de Boo, will contribute to this years edition with a presentation on the future of biochemicals. It will be during the bio-based chemical innovation session on Tuesday, May 15, at 2:00pm – 2:45pm.
The Cleantech Forum Europe, now in its 14th edition, continues to be the place to create connections and to connect the dots across multiple industrial sectors. This year’s Cleantech Forum Europe will be held in Antwerp, home to Europe’s largest chemical hub and the world’s most tightly concentrated petrochemical hub, and also home to Europe’s second largest port and a key continental logistics hub.
Happy to announce Prof Peter Punt is presenting our findings on protease regulatory systems research at Eurofung next month. Check it out in Berlin, Germany! March 19th-20th.